By Phyllis R. Brown, Eli Grushka
This thirty fourth quantity examines topics corresponding to high-performance capillary electrophoresis; gasoline chromatography, matrix isolation, and infrared spectrometry; and statistical theories of top overlap in chromatography.
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Extra resources for Advances in Chromatography, Volume 34
At the same time, the large-scale fractionation of human serum was optimized in recent years to meet the growing demand of specific proteins, such as immunoglobulins, clotting Page 10 factors, and so on. The extremely low concentration of some of these blood proteins gave rise to the need for improved fractionation and process control techniques. , factor VIII, interleukins) is essential, for even small impurities can lead to a total loss of therapeutic quality of the substance or cause an immune reaction.
Sample Preparation 10 B. Sample Injection 11 C. Protein Detection 15 D. Capillaries and Coatings 22 E. Separation of Serum and Plasma Proteins 25 III. Use of Ce in Serum and Plasma Protein Separation 35 A. Capillary Zone Electrophoresis 35 B. Capillary Isotachophoresis 39 C. Capillary Isoelectric Focusing 40 D. Capillary Gel Electrophoresis 42 E. Combined Separation Techniques 44 IV. Summary 44 Appendix A. Register of Human Proteins Separated by CE 46 Appendix B. Physical and Chemical Properties of Selected Human Serum and Plasma Proteins 47 Page 2 I Introduction The analysis of serum and plasma proteins is one of the most important tasks in clinical analysis, for a wide range of human diseases can be related to changes in the composition of the blood proteins.
In capillary isoelectric focusing (CIEF) the capillary ends are placed in two different electrolytes, specified as anolyte and catholyte buffer. The anolyte contains an acidic, the catholyte a basic solution. The capillary is filled with sample and a carefully designed mixture of ampholytes with varied IEPs, which in addition show a high buffer capacity at their IEP. As the electric field is applied, the ampholytes align themselves along the capillary according to their IEP, thus forming a steady pH gradient.