Analysis of Pharmaceuticals by Capillary Electrophoresis by Kevin D. Altria (auth.)

By Kevin D. Altria (auth.)

During the 1980's the research of prescribed drugs was once ruled via excessive Perfor­ mance Liquid Chromatography (HPLC). different separative suggestions equivalent to fuel Chromato­ graphy and skinny Layer Chromatography provided possible choices yet their quantitative capabili­ ties and/or solute diversity couldn't procedure that of HPLC. nearly all of prescription drugs are ionic and it might be average to imagine that electrophoresis will be beneficial within the research of prescription drugs. besides the fact that, the electrophore­ tic tools on hand within the 1980's have been labour extensive and hired post-separation detection approaches. through the overdue 1980's and early 1990's vast examine used to be con­ ducted into the chances of undertaking electrophoretic separations in capillaries. This method allowed online detection and will be played on totally automatic apparatus. This learn resulted in the appearance of recent day capillary electrophoresis (CE) tools which supply related functionality and automation degrees to that of HPLC. study was once additionally concerned with constructing purposes for CE and specific recognition used to be paid to purposes in the pharmaceutical research sector. those purposes proved that CE will be utilized to quite a lot of drug kinds together with water insoluble and impartial compounds. the facility to accomplish effective chiral separations of substances additionally elevated the recognition of the procedure. CE with oblique UV detection has develop into verified as an easy and powerful replacement to ion-exchange chromatography for the decision of small inorganic or natural ions.

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588 (1991) 335-339. 14. Wainwright A, Capillary electrophoresis applied to the analysis of pharmaceutical compounds, J. , 2 (1990) 166-175. References 19 15. Altria KD, Frake P, Gill I, Hadgett T, Kelly MA, and Rudd D R, Validated capillary electrophoresis method for the assay of a range of basic drugs and excipients, J. Pharm. Biomed. Analysis, 13 (1995) 951-957. 16. Gonzalez E and Laserna JJ, Capillary zone electrophoresis for the rapid screening of banned drugs in sport, Electrophoresis, 15 (1994) 240-243.

An additional approach to minimising integrator-related imprecision is to employ (52) a wider bore capillary which produces larger peaks, higher peak area counts and reduced integration errors. 7 Listing of factors affecting precision Comments Major considerations Internal standard Use whenever possible Constant temperature Always use High sample concentration Solubility considerations Injection time Avoid 1-2 seconds injection times Preconditioning injections Perform 2 blank injections Sample viscosity Match samples/standards Dissolving solvent Optimise during development Electrolyte composition Reduce depletion effects Minor considerations Buffer fluid levels Visually level Sample fluid levels Visually level Injection end of capillary Immerse in electrolyte vial Electrolyte co-injection Assess experimentally Voltage ramp Always use Delays following injection Minimise delays Constant current Assess experimentally Reproduced with permission from ref.

Pharm. Biomed. , 13 (1995) 505-509. 10. Bjornstdottir I and Hansen SH, Determination of opium alkaloids in opium by capillary electrophoresis, J. Pharm. Biomed. , 13 (1995) 687-693. II. Altria KD and Chanter YL, Validation of a capillary electrophoresis method for the determination of a quinolone antibiotic and its related impurities, J. , 652 (1993) 459-463. 12. Altria KD and Rogan MM, Reduction in sample pretreatment requirements by using highperformance capillary electrokinetic separation methods, J.

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