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Extra resources for Best Practice & Research Clinical Haematology Volume 24, Issue 1 March 2011
Hence, a local cue(s) must be operating, and must be provided at least in part by the transplanted, donor cells. In spite of the temporal link between bone formation and establishment of the hematopoietic microenvironment within heterotopic ossicles, the latter is not at all an obligate consequence of the former. Of note, bone marrow stromal cells from caPPR mice (constitutively active PTH/PTHrP receptor driven by the bone-speciﬁc type I collagen promoter , one of the two original disclosers of the osteoblastic niche ) are inefﬁcient in transferring the hematopoietic microenvironment.
3. Expression of selected markers on cultured MSC derived from sorted CD271brightCD56À and CD271brightCD56þ cells. Sorted MSC were cultured on gelatine-coated ﬂasks and cultured in b-FGF containing serum free medium. After reaching conﬂuence, cells were stained with antibodies against the indicated antigens. Note the rapid downregulation of CD56 and CD271 after culture and the upregulation of CD318. MSC with osteogenic differentiation potential. In contrast, only MSC from the CD56þ subset effectively gave rise to chondrocytes, suggesting that this subpopulation is the preferred source for therapeutic approaches in the ﬁeld of cartilage tissue repair .
In vitro" and multicolor phenotypic characterization of cell subpopulations identiﬁed in fresh human adipose tissue stromal vascular fraction and in the derived mesenchymal stem cells. J Transl Med 2007;5: 55.  Poloni A, Maurizi G, Rosini V, et al. Selection of CD271(þ) cells and human AB serum allows a large expansion of mesenchymal stromal cells from human bone marrow. Cytotherapy 2009;11:153–62.  Horn P, Bork S, Diehlmann A, et al. Isolation of human mesenchymal stromal cells is more efﬁcient by red blood cell lysis.